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4 x sds-page sample loading buffer  (Beyotime)


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    Structured Review

    Beyotime 4 x sds-page sample loading buffer
    4 X Sds Page Sample Loading Buffer, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4+%C3%97+sds+sample+loading+buffer/sds+loading+buffer/pmc07793474-122-19-24
    Average 90 stars, based on 1 article reviews
    4 x sds-page sample loading buffer - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    SDS Page:

    Article Title: Comprehensive role of prostate-specific antigen identified with proteomic analysis in prostate cancer.
    Article Snippet: Then, the supernatant was mixed with 1/4 volume of 5 × SDS-PAGE Sample Loading Buffer (Beyotime Biotechnology).

    Article Title: Liquiritigenin inhibits the migration, invasion, and EMT of prostate cancer through activating ER stress.
    Article Snippet: The rest of the supernatant was mixed with 1/4 volume of SDS-PAGE Sample Loading Buffer (5X; cat. no. P0015L; Beyotime Biotech Inc) and boiled for 10 min. After separating the total protein by 10 % sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE), the proteins were transferred to a nitrocellulose (NC) membrane.

    Article Title: Protective Effects of Liquiritigenin against Cisplatin-Induced Nephrotoxicity via NRF2/SIRT3-Mediated Improvement of Mitochondrial Function
    Article Snippet: The cell or tissue lysing reagents were collected, centrifuged at 14,000× g for 10 min at 4 °C, and mixed with 5 × SDS-PAGE Sample Loading Buffer (Beyotime Biotechnology, Shanghai, China).

    Article Title: Captopril alleviates glucocorticoid-induced osteonecrosis of the femoral head by mediating the ACE2/ Ang-(1-7)/Mas receptor cascade.
    Article Snippet: The renin–angiotensin–aldosterone system (RAAS) is locally expressed in skeletal tissue and is known to affect bone health.. This study investigated the therapeutic effects and potential mechanisms of the angiotensinconverting enzyme inhibitor (ACEI) captopril (CAP) in a rat model of glucocorticoid-induced femoral head necrosis (GIONFH).. Bone marrow mesenchymal stem cells (mBMSC) from mice treated with dexamethasone (DEX) in vitro and methylprednisolone (MPS)-induced rats in vivo were used to explore the effects and mechanisms of CAP, respectively.

    Article Title: NLRP1 restricts porcine deltacoronavirus infection via IL-11 inhibiting the phosphorylation of the ERK signaling pathway
    Article Snippet: The supernatant was separated by centrifugation at 14,000 rpm for 10 min at 4°C and boiled with 5× SDS-PAGE Sample Loading Buffer (Beyotime, China) for 10 min.

    Article Title: Iron-overload-induced ferroptosis in mouse cerebral toxoplasmosis promotes brain injury and could be inhibited by Deferiprone
    Article Snippet: The protein samples were prepared by diluting the samples to the same concentration with RIPA lysis buffer, adding 1∕4 volume of SDS-PAGE Sample Loading Buffer (5×, Beyotime Biotechnology, China), and heating at 100°C for 10 min.

    Article Title: Iron-overload-induced ferroptosis in mouse cerebral toxoplasmosis promotes brain injury and could be inhibited by Deferiprone.
    Article Snippet: The protein samples were prepared by diluting the samples to the same concentration with RIPA lysis buffer, adding 1/4 volume of SDS-PAGE Sample Loading Buffer (5×, Beyotime Biotechnology, China), and heating at 100 ̊C for 10 min.

    Article Title: Thyroid stimulating hormone aggravates diabetic retinopathy through the mitochondrial apoptotic pathway.
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: BJ‐2014‐066 Abstract Diabetic retinopathy (DR) is a common complication of diabetes mellitus.. High glucose‐induced mitochondrial apoptosis is involved in the loss of retinal pericytes (PCs), which is considered to be a predominant pathologic change of diabetic retinopathy (DR).. A high thyroid stimulating hormone (TSH) serum level is associated with an increased prevalence of DR in diabetic patients.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Comprehensive role of prostate-specific antigen identified with proteomic analysis in prostate cancer.
    Article Snippet: Then, the supernatant was mixed with 1/4 volume of 5 × SDS-PAGE Sample Loading Buffer (Beyotime Biotechnology).

    Article Title: Liquiritigenin inhibits the migration, invasion, and EMT of prostate cancer through activating ER stress.
    Article Snippet: The rest of the supernatant was mixed with 1/4 volume of SDS-PAGE Sample Loading Buffer (5X; cat. no. P0015L; Beyotime Biotech Inc) and boiled for 10 min. After separating the total protein by 10 % sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE), the proteins were transferred to a nitrocellulose (NC) membrane.

    Article Title: Protective Effects of Liquiritigenin against Cisplatin-Induced Nephrotoxicity via NRF2/SIRT3-Mediated Improvement of Mitochondrial Function
    Article Snippet: The cell or tissue lysing reagents were collected, centrifuged at 14,000× g for 10 min at 4 °C, and mixed with 5 × SDS-PAGE Sample Loading Buffer (Beyotime Biotechnology, Shanghai, China).

    Article Title: Captopril alleviates glucocorticoid-induced osteonecrosis of the femoral head by mediating the ACE2/ Ang-(1-7)/Mas receptor cascade.
    Article Snippet: The renin–angiotensin–aldosterone system (RAAS) is locally expressed in skeletal tissue and is known to affect bone health.. This study investigated the therapeutic effects and potential mechanisms of the angiotensinconverting enzyme inhibitor (ACEI) captopril (CAP) in a rat model of glucocorticoid-induced femoral head necrosis (GIONFH).. Bone marrow mesenchymal stem cells (mBMSC) from mice treated with dexamethasone (DEX) in vitro and methylprednisolone (MPS)-induced rats in vivo were used to explore the effects and mechanisms of CAP, respectively.

    Article Title: NLRP1 restricts porcine deltacoronavirus infection via IL-11 inhibiting the phosphorylation of the ERK signaling pathway
    Article Snippet: The supernatant was separated by centrifugation at 14,000 rpm for 10 min at 4°C and boiled with 5× SDS-PAGE Sample Loading Buffer (Beyotime, China) for 10 min.

    Article Title: Iron-overload-induced ferroptosis in mouse cerebral toxoplasmosis promotes brain injury and could be inhibited by Deferiprone
    Article Snippet: The protein samples were prepared by diluting the samples to the same concentration with RIPA lysis buffer, adding 1∕4 volume of SDS-PAGE Sample Loading Buffer (5×, Beyotime Biotechnology, China), and heating at 100°C for 10 min.

    Article Title: Iron-overload-induced ferroptosis in mouse cerebral toxoplasmosis promotes brain injury and could be inhibited by Deferiprone.
    Article Snippet: The protein samples were prepared by diluting the samples to the same concentration with RIPA lysis buffer, adding 1/4 volume of SDS-PAGE Sample Loading Buffer (5×, Beyotime Biotechnology, China), and heating at 100 ̊C for 10 min.

    Article Title: Thyroid stimulating hormone aggravates diabetic retinopathy through the mitochondrial apoptotic pathway.
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: BJ‐2014‐066 Abstract Diabetic retinopathy (DR) is a common complication of diabetes mellitus.. High glucose‐induced mitochondrial apoptosis is involved in the loss of retinal pericytes (PCs), which is considered to be a predominant pathologic change of diabetic retinopathy (DR).. A high thyroid stimulating hormone (TSH) serum level is associated with an increased prevalence of DR in diabetic patients.

    Membrane:

    Article Title: Comprehensive role of prostate-specific antigen identified with proteomic analysis in prostate cancer.
    Article Snippet: Then, the supernatant was mixed with 1/4 volume of 5 × SDS-PAGE Sample Loading Buffer (Beyotime Biotechnology).

    Article Title: Liquiritigenin inhibits the migration, invasion, and EMT of prostate cancer through activating ER stress.
    Article Snippet: The rest of the supernatant was mixed with 1/4 volume of SDS-PAGE Sample Loading Buffer (5X; cat. no. P0015L; Beyotime Biotech Inc) and boiled for 10 min. After separating the total protein by 10 % sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE), the proteins were transferred to a nitrocellulose (NC) membrane.

    Article Title: Protective Effects of Liquiritigenin against Cisplatin-Induced Nephrotoxicity via NRF2/SIRT3-Mediated Improvement of Mitochondrial Function
    Article Snippet: The cell or tissue lysing reagents were collected, centrifuged at 14,000× g for 10 min at 4 °C, and mixed with 5 × SDS-PAGE Sample Loading Buffer (Beyotime Biotechnology, Shanghai, China).

    Article Title: Captopril alleviates glucocorticoid-induced osteonecrosis of the femoral head by mediating the ACE2/ Ang-(1-7)/Mas receptor cascade.
    Article Snippet: The renin–angiotensin–aldosterone system (RAAS) is locally expressed in skeletal tissue and is known to affect bone health.. This study investigated the therapeutic effects and potential mechanisms of the angiotensinconverting enzyme inhibitor (ACEI) captopril (CAP) in a rat model of glucocorticoid-induced femoral head necrosis (GIONFH).. Bone marrow mesenchymal stem cells (mBMSC) from mice treated with dexamethasone (DEX) in vitro and methylprednisolone (MPS)-induced rats in vivo were used to explore the effects and mechanisms of CAP, respectively.

    Article Title: NLRP1 restricts porcine deltacoronavirus infection via IL-11 inhibiting the phosphorylation of the ERK signaling pathway
    Article Snippet: The supernatant was separated by centrifugation at 14,000 rpm for 10 min at 4°C and boiled with 5× SDS-PAGE Sample Loading Buffer (Beyotime, China) for 10 min.

    Article Title: Iron-overload-induced ferroptosis in mouse cerebral toxoplasmosis promotes brain injury and could be inhibited by Deferiprone
    Article Snippet: The protein samples were prepared by diluting the samples to the same concentration with RIPA lysis buffer, adding 1∕4 volume of SDS-PAGE Sample Loading Buffer (5×, Beyotime Biotechnology, China), and heating at 100°C for 10 min.

    Article Title: Iron-overload-induced ferroptosis in mouse cerebral toxoplasmosis promotes brain injury and could be inhibited by Deferiprone.
    Article Snippet: The protein samples were prepared by diluting the samples to the same concentration with RIPA lysis buffer, adding 1/4 volume of SDS-PAGE Sample Loading Buffer (5×, Beyotime Biotechnology, China), and heating at 100 ̊C for 10 min.

    Article Title: Thyroid stimulating hormone aggravates diabetic retinopathy through the mitochondrial apoptotic pathway.
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: BJ‐2014‐066 Abstract Diabetic retinopathy (DR) is a common complication of diabetes mellitus.. High glucose‐induced mitochondrial apoptosis is involved in the loss of retinal pericytes (PCs), which is considered to be a predominant pathologic change of diabetic retinopathy (DR).. A high thyroid stimulating hormone (TSH) serum level is associated with an increased prevalence of DR in diabetic patients.

    Centrifugation:

    Article Title: Comprehensive role of prostate-specific antigen identified with proteomic analysis in prostate cancer.
    Article Snippet: Then, the supernatant was mixed with 1/4 volume of 5 × SDS-PAGE Sample Loading Buffer (Beyotime Biotechnology).

    Article Title: Liquiritigenin inhibits the migration, invasion, and EMT of prostate cancer through activating ER stress.
    Article Snippet: The rest of the supernatant was mixed with 1/4 volume of SDS-PAGE Sample Loading Buffer (5X; cat. no. P0015L; Beyotime Biotech Inc) and boiled for 10 min. After separating the total protein by 10 % sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE), the proteins were transferred to a nitrocellulose (NC) membrane.

    Article Title: Protective Effects of Liquiritigenin against Cisplatin-Induced Nephrotoxicity via NRF2/SIRT3-Mediated Improvement of Mitochondrial Function
    Article Snippet: The cell or tissue lysing reagents were collected, centrifuged at 14,000× g for 10 min at 4 °C, and mixed with 5 × SDS-PAGE Sample Loading Buffer (Beyotime Biotechnology, Shanghai, China).

    Article Title: Captopril alleviates glucocorticoid-induced osteonecrosis of the femoral head by mediating the ACE2/ Ang-(1-7)/Mas receptor cascade.
    Article Snippet: The renin–angiotensin–aldosterone system (RAAS) is locally expressed in skeletal tissue and is known to affect bone health.. This study investigated the therapeutic effects and potential mechanisms of the angiotensinconverting enzyme inhibitor (ACEI) captopril (CAP) in a rat model of glucocorticoid-induced femoral head necrosis (GIONFH).. Bone marrow mesenchymal stem cells (mBMSC) from mice treated with dexamethasone (DEX) in vitro and methylprednisolone (MPS)-induced rats in vivo were used to explore the effects and mechanisms of CAP, respectively.

    Article Title: NLRP1 restricts porcine deltacoronavirus infection via IL-11 inhibiting the phosphorylation of the ERK signaling pathway
    Article Snippet: The supernatant was separated by centrifugation at 14,000 rpm for 10 min at 4°C and boiled with 5× SDS-PAGE Sample Loading Buffer (Beyotime, China) for 10 min.

    Article Title: Iron-overload-induced ferroptosis in mouse cerebral toxoplasmosis promotes brain injury and could be inhibited by Deferiprone
    Article Snippet: The protein samples were prepared by diluting the samples to the same concentration with RIPA lysis buffer, adding 1∕4 volume of SDS-PAGE Sample Loading Buffer (5×, Beyotime Biotechnology, China), and heating at 100°C for 10 min.

    Article Title: Iron-overload-induced ferroptosis in mouse cerebral toxoplasmosis promotes brain injury and could be inhibited by Deferiprone.
    Article Snippet: The protein samples were prepared by diluting the samples to the same concentration with RIPA lysis buffer, adding 1/4 volume of SDS-PAGE Sample Loading Buffer (5×, Beyotime Biotechnology, China), and heating at 100 ̊C for 10 min.

    Article Title: Thyroid stimulating hormone aggravates diabetic retinopathy through the mitochondrial apoptotic pathway.
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: BJ‐2014‐066 Abstract Diabetic retinopathy (DR) is a common complication of diabetes mellitus.. High glucose‐induced mitochondrial apoptosis is involved in the loss of retinal pericytes (PCs), which is considered to be a predominant pathologic change of diabetic retinopathy (DR).. A high thyroid stimulating hormone (TSH) serum level is associated with an increased prevalence of DR in diabetic patients.

    Concentration Assay:

    Article Title: Comprehensive role of prostate-specific antigen identified with proteomic analysis in prostate cancer.
    Article Snippet: Then, the supernatant was mixed with 1/4 volume of 5 × SDS-PAGE Sample Loading Buffer (Beyotime Biotechnology).

    Article Title: Liquiritigenin inhibits the migration, invasion, and EMT of prostate cancer through activating ER stress.
    Article Snippet: The rest of the supernatant was mixed with 1/4 volume of SDS-PAGE Sample Loading Buffer (5X; cat. no. P0015L; Beyotime Biotech Inc) and boiled for 10 min. After separating the total protein by 10 % sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE), the proteins were transferred to a nitrocellulose (NC) membrane.

    Article Title: Protective Effects of Liquiritigenin against Cisplatin-Induced Nephrotoxicity via NRF2/SIRT3-Mediated Improvement of Mitochondrial Function
    Article Snippet: The cell or tissue lysing reagents were collected, centrifuged at 14,000× g for 10 min at 4 °C, and mixed with 5 × SDS-PAGE Sample Loading Buffer (Beyotime Biotechnology, Shanghai, China).

    Article Title: Captopril alleviates glucocorticoid-induced osteonecrosis of the femoral head by mediating the ACE2/ Ang-(1-7)/Mas receptor cascade.
    Article Snippet: The renin–angiotensin–aldosterone system (RAAS) is locally expressed in skeletal tissue and is known to affect bone health.. This study investigated the therapeutic effects and potential mechanisms of the angiotensinconverting enzyme inhibitor (ACEI) captopril (CAP) in a rat model of glucocorticoid-induced femoral head necrosis (GIONFH).. Bone marrow mesenchymal stem cells (mBMSC) from mice treated with dexamethasone (DEX) in vitro and methylprednisolone (MPS)-induced rats in vivo were used to explore the effects and mechanisms of CAP, respectively.

    Article Title: NLRP1 restricts porcine deltacoronavirus infection via IL-11 inhibiting the phosphorylation of the ERK signaling pathway
    Article Snippet: The supernatant was separated by centrifugation at 14,000 rpm for 10 min at 4°C and boiled with 5× SDS-PAGE Sample Loading Buffer (Beyotime, China) for 10 min.

    Article Title: Iron-overload-induced ferroptosis in mouse cerebral toxoplasmosis promotes brain injury and could be inhibited by Deferiprone
    Article Snippet: The protein samples were prepared by diluting the samples to the same concentration with RIPA lysis buffer, adding 1∕4 volume of SDS-PAGE Sample Loading Buffer (5×, Beyotime Biotechnology, China), and heating at 100°C for 10 min.

    Article Title: Iron-overload-induced ferroptosis in mouse cerebral toxoplasmosis promotes brain injury and could be inhibited by Deferiprone.
    Article Snippet: The protein samples were prepared by diluting the samples to the same concentration with RIPA lysis buffer, adding 1/4 volume of SDS-PAGE Sample Loading Buffer (5×, Beyotime Biotechnology, China), and heating at 100 ̊C for 10 min.

    Article Title: Thyroid stimulating hormone aggravates diabetic retinopathy through the mitochondrial apoptotic pathway.
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: BJ‐2014‐066 Abstract Diabetic retinopathy (DR) is a common complication of diabetes mellitus.. High glucose‐induced mitochondrial apoptosis is involved in the loss of retinal pericytes (PCs), which is considered to be a predominant pathologic change of diabetic retinopathy (DR).. A high thyroid stimulating hormone (TSH) serum level is associated with an increased prevalence of DR in diabetic patients.

    Lysis:

    Article Title: Comprehensive role of prostate-specific antigen identified with proteomic analysis in prostate cancer.
    Article Snippet: Then, the supernatant was mixed with 1/4 volume of 5 × SDS-PAGE Sample Loading Buffer (Beyotime Biotechnology).

    Article Title: Liquiritigenin inhibits the migration, invasion, and EMT of prostate cancer through activating ER stress.
    Article Snippet: The rest of the supernatant was mixed with 1/4 volume of SDS-PAGE Sample Loading Buffer (5X; cat. no. P0015L; Beyotime Biotech Inc) and boiled for 10 min. After separating the total protein by 10 % sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE), the proteins were transferred to a nitrocellulose (NC) membrane.

    Article Title: Protective Effects of Liquiritigenin against Cisplatin-Induced Nephrotoxicity via NRF2/SIRT3-Mediated Improvement of Mitochondrial Function
    Article Snippet: The cell or tissue lysing reagents were collected, centrifuged at 14,000× g for 10 min at 4 °C, and mixed with 5 × SDS-PAGE Sample Loading Buffer (Beyotime Biotechnology, Shanghai, China).

    Article Title: Captopril alleviates glucocorticoid-induced osteonecrosis of the femoral head by mediating the ACE2/ Ang-(1-7)/Mas receptor cascade.
    Article Snippet: The renin–angiotensin–aldosterone system (RAAS) is locally expressed in skeletal tissue and is known to affect bone health.. This study investigated the therapeutic effects and potential mechanisms of the angiotensinconverting enzyme inhibitor (ACEI) captopril (CAP) in a rat model of glucocorticoid-induced femoral head necrosis (GIONFH).. Bone marrow mesenchymal stem cells (mBMSC) from mice treated with dexamethasone (DEX) in vitro and methylprednisolone (MPS)-induced rats in vivo were used to explore the effects and mechanisms of CAP, respectively.

    Article Title: NLRP1 restricts porcine deltacoronavirus infection via IL-11 inhibiting the phosphorylation of the ERK signaling pathway
    Article Snippet: The supernatant was separated by centrifugation at 14,000 rpm for 10 min at 4°C and boiled with 5× SDS-PAGE Sample Loading Buffer (Beyotime, China) for 10 min.

    Article Title: Iron-overload-induced ferroptosis in mouse cerebral toxoplasmosis promotes brain injury and could be inhibited by Deferiprone
    Article Snippet: The protein samples were prepared by diluting the samples to the same concentration with RIPA lysis buffer, adding 1∕4 volume of SDS-PAGE Sample Loading Buffer (5×, Beyotime Biotechnology, China), and heating at 100°C for 10 min.

    Article Title: Iron-overload-induced ferroptosis in mouse cerebral toxoplasmosis promotes brain injury and could be inhibited by Deferiprone.
    Article Snippet: The protein samples were prepared by diluting the samples to the same concentration with RIPA lysis buffer, adding 1/4 volume of SDS-PAGE Sample Loading Buffer (5×, Beyotime Biotechnology, China), and heating at 100 ̊C for 10 min.

    Article Title: Thyroid stimulating hormone aggravates diabetic retinopathy through the mitochondrial apoptotic pathway.
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: BJ‐2014‐066 Abstract Diabetic retinopathy (DR) is a common complication of diabetes mellitus.. High glucose‐induced mitochondrial apoptosis is involved in the loss of retinal pericytes (PCs), which is considered to be a predominant pathologic change of diabetic retinopathy (DR).. A high thyroid stimulating hormone (TSH) serum level is associated with an increased prevalence of DR in diabetic patients.



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    Image Search Results


    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Loss of Cathepsin K Impairs Collagen Biogenesis and Enhances Actin Polymerization in Trabecular Meshwork

    doi: 10.1167/iovs.67.1.63

    Figure Lengend Snippet: Materials and Reagents Used

    Article Snippet: 4× SDS sample loading buffer , J63615 , Thermo Fisher Scientific.

    Techniques: Knockdown, Control, Membrane, Bicinchoninic Acid Protein Assay, Pore Size, Staining, Western Blot, Recombinant, Mass Spectrometry, Colorimetric Assay, In Vivo